Fig 1: Concentration of circulating TNFα, IL1β and IL18 in oviduct tissue of P. sinensis.The values (*) obtained in July were significantly higher (P < 0.05) than those obtained in January.
Fig 2: mRNA expression levels of cell proliferation markers in host cells incubated with growth media containing IL-18 and IFN-γ for different time intervals. (A) Ki67 and (B) PCNA. Host cells incubated with growth medium were used as a control. The target mRNA expression was normalised to the expression of GAPDH in the same sample. At least two independent experiments were performed. Data are presented as the mean ± SD from triplicate cultures. *P<0.05, **P<0.01 and ***P<0.001 vs. day 4. d, day; PCNA, proliferating cell nuclear antigen.
Fig 3: Cytokine levels in conditioned media of HFFs parasitized with RH and ME49 strains of T. gondii. (A) IL-1β, (B) IL-10, (C) IL-18 and (D) IFN-γ. The cytokine level was calculated as the ratio of the experimental value (pg/ml) relative to the value in non-infected host cells. At least two independent experiments were performed. Data are presented as the mean ± SD from triplicate cultures. *P<0.05 and **P<0.01 vs. day 2.
Fig 4: Soluble factor levels in conditioned media of IL-21-silenced HCT116 cells. (A) IL-18 and (B) IFN-γ. Conditioned media of non-parasitized host cells were used as a control. The cytokine level was calculated as the ratio of the experimental value (pg/ml) relative to that of the control. At least two independent experiments were performed. Data are presented as the mean ± SD from triplicate cultures. **P<0.01 and ***P<0.001 vs. day 1. d, day.
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